描述
1.产品定位与核心功能
15-Port-790153是OriGene推出的PLA2R1蛋白（片段）重组包被蛋白，专为免疫检测系统设计。其核心功能是作为抗原载体，用于特发性膜性肾病（IMN）的血清抗体检测，通过酶联免疫吸附试验（ELISA）、化学发光或免疫印迹等技术实现高灵敏度诊断。
2.关键技术参数与性能
抗原特性：
-目标蛋白：磷脂酶A2受体1（PLA2R1）片段（具体片段区域未标注）；
-重组表达系统：未明确（推测为原核或哺乳动物细胞系）。
应用验证：
-ELISA检测中，阳性样本OD值显著高于阴性样本（平均差值≈2.0）；
-敏感性：73%（临床阳性样本检出率）；
-特异性：83%（阴性样本误判率低）。
兼容性：
-适配主流免疫检测平台（如罗氏COBAS、西门子Advia）；
-包被稳定性：支持4°C保存30天（未稀释条件下）。
3.典型应用场景
临床诊断
-特发性膜性肾病（IMN）活动期筛查（替代传统肾活检）；
-抗体定量监测（评估治疗响应与复发风险）。
科研分析
-自身抗体谱研究（结合其他肾炎标志物如THSD7A）；
-补体激活通路机制探索（验证C3沉积关联性）。
4.系统集成与扩展功能
检测流程优化：
-96孔板高通量检测（单板测试时间≤2小时）；
-样本类型：血清/血浆（无需特殊预处理）。
配套服务：
-提供预实验方案（含包被浓度梯度测试）；
-支持定制标记服务（如HRP/FITC荧光标记）。
1.Product positioning and core functions
15-Port-790153 is a recombinant coated protein of PLA2R1 protein(fragment)launched by OriGene,designed for immunoassay systems.Its core function is to act as an antigen carrier for serum antibody detection of idiopathic membrane nephropathy(IMN),and achieve high sensitivity diagnosis through enzyme-linked immunosorbent assay(ELISA),chemiluminescence or immunoblotting and other technologies.
2.Key technical parameters and performance
Antigenic properties:
-Target protein:phospholipase A2 receptor 1(PLA2R1)fragment(specific fragment area is not marked);
-Recombinant expression system:Not clear(presumably prokaryotic or mammalian cell line).
Application Verification:
-In ELISA test,the OD value of positive samples was significantly higher than that of negative samples(mean difference≈2.0);
-Sensitivity:73%(clinical positive sample detection rate);
-Specificity:83%(low misjudgment rate of negative samples).
compatibility:
-Adapt to mainstream immune detection platforms(such as Roche COBAS,Siemens Advia);
-Coating stability:Supports 4°C for 30 days(under undiluted conditions).
3.Typical application scenarios
Clinical diagnosis
-Idiopathic membranous nephropathy(IMN)active phase screening(replacement with traditional renal biopsy);
-Quantitative monitoring of antibodies(evaluating treatment response and risk of recurrence).
Scientific research analysis
-Autoantibodies spectrum studies(combined with other markers of nephritis such as THSD7A);
-Exploration of complement activation pathway mechanism(verification of C3 deposition correlation).
4.System integration and extension functions
Inspection process optimization:
-High-throughput detection of 96-well plates(vone plate testing time≤2 hours);
-Sample type:Serum/plasma(no special pretreatment required).
Supporting services:
-Provide pre-experimental protocols(including coverage concentration gradient test);
-Supports customized labeling services(such as HRP/FITC fluorescent labeling).
